Coding
His-Sc_Si4

Part:BBa_K1602030

Designed by: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder   Group: iGEM15_TU_Darmstadt   (2015-09-16)

HisTag-Scaffold_Si4-Tag fusion protein

This part codes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 22
    Illegal BamHI site found at 877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None